Transcriptome analysis of tobacco BY-2 cells elicited by cryptogein reveals new potential actors of calcium-dependent and calcium-independent plant defense pathways.
Identifieur interne : 001414 ( Main/Exploration ); précédent : 001413; suivant : 001415Transcriptome analysis of tobacco BY-2 cells elicited by cryptogein reveals new potential actors of calcium-dependent and calcium-independent plant defense pathways.
Auteurs : Nicolas Amelot [France] ; François Dorlhac De Borne ; Hélène San Clemente ; Christian Mazars ; Jacqueline Grima-Pettenati ; Christian BrièreSource :
- Cell calcium [ 1532-1991 ] ; 2012.
Descripteurs français
- KwdFr :
- Calcium (métabolisme), Cellules végétales (MeSH), Génome végétal (MeSH), Maladies des plantes (MeSH), Phytophthora (MeSH), Protéines d'algue (MeSH), Protéines fongiques (MeSH), Régulation de l'expression des gènes végétaux (MeSH), Signalisation calcique (MeSH), Tabac (cytologie), Tabac (microbiologie), Tabac (métabolisme), Transcriptome (MeSH).
- MESH :
- cytologie : Tabac.
- microbiologie : Tabac.
- métabolisme : Calcium, Tabac.
- Cellules végétales, Génome végétal, Maladies des plantes, Phytophthora, Protéines d'algue, Protéines fongiques, Régulation de l'expression des gènes végétaux, Signalisation calcique, Transcriptome.
English descriptors
- KwdEn :
- Algal Proteins (MeSH), Calcium (metabolism), Calcium Signaling (MeSH), Fungal Proteins (MeSH), Gene Expression Regulation, Plant (MeSH), Genome, Plant (MeSH), Phytophthora (MeSH), Plant Cells (MeSH), Plant Diseases (MeSH), Tobacco (cytology), Tobacco (metabolism), Tobacco (microbiology), Transcriptome (MeSH).
- MESH :
- chemical , metabolism : Calcium.
- chemical : Algal Proteins, Fungal Proteins.
- cytology : Tobacco.
- metabolism : Tobacco.
- microbiology : Tobacco.
- Calcium Signaling, Gene Expression Regulation, Plant, Genome, Plant, Phytophthora, Plant Cells, Plant Diseases, Transcriptome.
Abstract
Cryptogein is a proteinaceous elicitor secreted by the oomycete Phytophthora cryptogea, which induces a hypersensitive response in tobacco plants. We have previously reported that in tobacco BY-2 cells treated with cryptogein, most of the genes of the phenylpropanoid pathway were upregulated and cell wall-bound phenolics accumulated. Both events were Ca(2+) dependent. In this study, we designed a microarray covering a large proportion of the tobacco genome and monitored gene expression in cryptogein-elicited BY-2 cells to get a more complete view of the transcriptome changes and to assess their Ca(2+) dependence. The predominant functional gene categories affected by cryptogein included stress- and disease-related proteins, phenylpropanoid pathway, signaling components, transcription factors and cell wall reinforcement. Among the 3819 unigenes whose expression changed more than fourfold, 90% were Ca(2+) dependent, as determined by their sensitivity to lanthanum chloride. The most Ca(2+)-dependent transcripts upregulated by cryptogein were involved in defense responses or the oxylipin pathway. This genome-wide study strongly supports the importance of Ca(2+)-dependent transcriptional regulation of regulatory and defense-related genes contributing to cryptogein responses in tobacco.
DOI: 10.1016/j.ceca.2011.11.010
PubMed: 22177386
Affiliations:
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(MeSH)</term><term>Protéines fongiques (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Signalisation calcique (MeSH)</term><term>Tabac (cytologie)</term><term>Tabac (microbiologie)</term><term>Tabac (métabolisme)</term><term>Transcriptome (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Calcium</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Algal Proteins</term><term>Fungal Proteins</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Tobacco</term></keywords><keywords 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We have previously reported that in tobacco BY-2 cells treated with cryptogein, most of the genes of the phenylpropanoid pathway were upregulated and cell wall-bound phenolics accumulated. Both events were Ca(2+) dependent. In this study, we designed a microarray covering a large proportion of the tobacco genome and monitored gene expression in cryptogein-elicited BY-2 cells to get a more complete view of the transcriptome changes and to assess their Ca(2+) dependence. The predominant functional gene categories affected by cryptogein included stress- and disease-related proteins, phenylpropanoid pathway, signaling components, transcription factors and cell wall reinforcement. Among the 3819 unigenes whose expression changed more than fourfold, 90% were Ca(2+) dependent, as determined by their sensitivity to lanthanum chloride. The most Ca(2+)-dependent transcripts upregulated by cryptogein were involved in defense responses or the oxylipin pathway. This genome-wide study strongly supports the importance of Ca(2+)-dependent transcriptional regulation of regulatory and defense-related genes contributing to cryptogein responses in tobacco.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">22177386</PMID><DateCompleted><Year>2012</Year><Month>06</Month><Day>08</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1532-1991</ISSN><JournalIssue CitedMedium="Internet"><Volume>51</Volume><Issue>2</Issue><PubDate><Year>2012</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Cell calcium</Title><ISOAbbreviation>Cell Calcium</ISOAbbreviation></Journal><ArticleTitle>Transcriptome analysis of tobacco BY-2 cells elicited by cryptogein reveals new potential actors of calcium-dependent and calcium-independent plant defense pathways.</ArticleTitle><Pagination><MedlinePgn>117-30</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ceca.2011.11.010</ELocationID><Abstract><AbstractText>Cryptogein is a proteinaceous elicitor secreted by the oomycete Phytophthora cryptogea, which induces a hypersensitive response in tobacco plants. We have previously reported that in tobacco BY-2 cells treated with cryptogein, most of the genes of the phenylpropanoid pathway were upregulated and cell wall-bound phenolics accumulated. Both events were Ca(2+) dependent. In this study, we designed a microarray covering a large proportion of the tobacco genome and monitored gene expression in cryptogein-elicited BY-2 cells to get a more complete view of the transcriptome changes and to assess their Ca(2+) dependence. The predominant functional gene categories affected by cryptogein included stress- and disease-related proteins, phenylpropanoid pathway, signaling components, transcription factors and cell wall reinforcement. Among the 3819 unigenes whose expression changed more than fourfold, 90% were Ca(2+) dependent, as determined by their sensitivity to lanthanum chloride. The most Ca(2+)-dependent transcripts upregulated by cryptogein were involved in defense responses or the oxylipin pathway. This genome-wide study strongly supports the importance of Ca(2+)-dependent transcriptional regulation of regulatory and defense-related genes contributing to cryptogein responses in tobacco.</AbstractText><CopyrightInformation>Copyright © 2011 Elsevier Ltd. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Amelot</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Université de Toulouse, Laboratoire de Recherches en Sciences Végétales, Castanet-Tolosan, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dorlhac de Borne</LastName><ForeName>François</ForeName><Initials>F</Initials></Author><Author ValidYN="Y"><LastName>San Clemente</LastName><ForeName>Hélène</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Mazars</LastName><ForeName>Christian</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Grima-Pettenati</LastName><ForeName>Jacqueline</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Brière</LastName><ForeName>Christian</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType 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Borne">François Dorlhac De Borne</name><name sortKey="Grima Pettenati, Jacqueline" sort="Grima Pettenati, Jacqueline" uniqKey="Grima Pettenati J" first="Jacqueline" last="Grima-Pettenati">Jacqueline Grima-Pettenati</name><name sortKey="Mazars, Christian" sort="Mazars, Christian" uniqKey="Mazars C" first="Christian" last="Mazars">Christian Mazars</name><name sortKey="San Clemente, Helene" sort="San Clemente, Helene" uniqKey="San Clemente H" first="Hélène" last="San Clemente">Hélène San Clemente</name></noCountry><country name="France"><noRegion><name sortKey="Amelot, Nicolas" sort="Amelot, Nicolas" uniqKey="Amelot N" first="Nicolas" last="Amelot">Nicolas Amelot</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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